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myocyte growth medium (PromoCell)

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PromoCell myocyte growth medium
Myocyte Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/myocyte+medium/Myocyte+Growth+Medium/pmc13194094-38-9-13
Average 95 stars, based on 85 article reviews

myocyte growth medium - by Bioz Stars, 2026-09

95/100 stars

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Article Title: <i>Cistanche tubulosa</i> Extract Protects Cast Immobilization‐Induced Declines of Muscle Strength and Muscle Mass
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C ons L icense 4 of 7 Traditional & Kampo Medicine, 2026 CaCl2 for 1 h. The obtained myocytes were grown in Myocyte Medium (Promo Cell, Heidelberg, Germany) with poly D- lysine coating at 1.45 or 7.25 × 104 cells/cm2 in 8- well chamber slides (Corning, Corning, NY, USA).

Isolation:

Article Title: Acteoside Improves Muscle Atrophy and Motor Function by Inducing New Myokine Secretion in Chronic Spinal Cord Injury
Article Snippet: .. Isolated skeletal muscle from the whole hindlimb was minced, then dissociated in HBSS containing 1 mg/mL collagenase (Sigma-Aldrich), 0.02 mg/mL dispase I (Sigma-Aldrich), and 2.5 mM CaCl 2 for 1 h. The obtained myocytes were grown in Myocyte Medium (Promo Cell, Heidelberg, Germany) in several culture plates without coating; cells were plated at a cell density of 1.0 × 10 cells/well in white-walled 96-well plates (Greiner Japan, Tokyo, Japan), 2.0 × 10 cells/well in eight-well chamber slides, or 6.0 × 10 cells/dish in 100-mm dishes (Corning). ..

Article Title: Oral Treatment of Water‐Soluble Rutin Protects Against Cast Immobilisation–Induced Cognitive Impairment
Article Snippet: .. The skeletal muscle isolated from the whole hind limb was minced and dissociated in HBSS containing 480 U/ mL collagenase (FUJIFILM Wako Pure Chemical) and 2.5 mM CaCl2 for 1 h. The obtained myocytes were grown in Myocyte Medium (Promo Cell, Heidelberg, Germany) with poly Dlysine coating at 3.68 × 104 cells/cm2 in eight- well chamber slides (Corning). .. After drug treatment, myocytes were fixed with 4% paraformaldehyde for 30 min and immunostained at 4 °C for 24 h in 0.3% Triton X- PBS with the following primary antibodies: mouse anti–myosin heavy chain (MHC; 1:500, #05- 716- I, Merck) and rabbit anti- ATP6 (1:100, #55313- 1- AP, Proteintech, Rosemont, IL, USA).

Recombinant:

Article Title: Integrated heart/cancer on a chip to reproduce the side effects of anti-cancer drugs in vitro
Article Snippet: .. Human primary cardiomyocytes (hCMs) were obtained from PromoCell, and were maintained in myocyte medium (PromoCell) supplemented with 0.05% (v/v) fetal calf serum (PromoCell), 0.5 ng mL 1 recombinant human epidermal growth factor (PromoCell), 2 ng mL 1 recombinant human basic broblast growth factor (PromoCell), and 5 mg mL 1 recombinant human insulin (PromoCell) in a humidied incubator at 37 C and 5% (v/v) CO2. .. Cell culture medium was changed every three days, and the cells were passaged using a trypsin/ ethylenediaminetetraacetic acid (EDTA) (0.04%/0.03% [v/v]) solution (Thermo Fisher Scientic) every week.